TLR4 and infection: To that end, we stimulated different TLRs with their known ligands (e.g. TLR2 was stimulated with Pam3Csk4; TLR4 was stimulated with LPS, TLR7 was stimulated with imiquimod and TLR9 was stimulated with CpG-DNA) in the presence or absence of stimulation with Rv2463 or infection with M. tb H37Rv.