Observed versus expected genotype frequencies were calculated and no deviation from Hardy–Weinberg equilibrium was observed, except for the TGFB1+869T>C polymorphism in patients group (EGF+61G>A: RCC group, P = 0.073, control group, P = 0.078; TGFB1+869T>C: RCC group, P = 0.023, control group, P = 0.195). This evidence concerns the gene EGF and renal cell carcinoma.