The results revealed that cyclin A2 (CCNA2), cyclin E1 (CCNE1), and E2F transcription factor 2 (E2F2) were significantly increased in the transgenic HCCs and could be upregulated by pre-S2 mutant through MYC activation in HCC cells (S3 Fig). The gene discussed is CCNE1; the disease is hepatocellular carcinoma.