Our study showed that CDC25A is a direct target of let-7c in HCC; cellular CDC25A expression was decreased at the protein level through transfection with let-7c, which directly bound to the 3′UTR, but not at the mRNA level, indicating that let-7c functions by regulating the protein level of CDC25A mainly at the post-transcriptional level. Here, CDC25A is linked to hepatocellular carcinoma.