Next, we measured the relative mRNA and protein levels of OGFOD1 in a nontransformed mammary epithelial cell line, MCF-10A (control cell line) and breast cancer cell lines, MCF-7 / T47D / ZR-75-1 (luminal A, ER+/PR+/–/HER2−), MDA-MB-231 / MDA-MB-468 / BT-20 (triple-negative, ER/PR/HER2−), MDA-MB-453 (HER2, ER−/PR−/HER2+). This evidence concerns the gene OGFOD1 and breast cancer.