To further evaluate whether CXCR6 induces tumor metastasis in vivo, we transplanted MDA-231-lucCXCR6/shCXCR6-2# and respective MDA-231-lucCXCR6/shCtrl cells tagged with luciferase into nude balb/c mice by tail vein injection. This evidence concerns the gene CXCR6 and neoplasm.