Among these genes, HMGA2, CLDN1, TFPI2, KIAA0101 and EGR1 are cancer related genes according to Diseases Association Analysis and increased expression of EGR1 was confirmed by further semi-quantitative RT-PCR, quantitative RT-PCR and western blot in BCL6B re-expressed HepG2 and SNU449 cells. The gene discussed is CLDN1; the disease is cancer.