ESR1 and breast carcinoma: To investigate the role of preadipocytes and adipocytes, we performed ADCC assays on HER2-positive (HER2+) estrogen receptor (ER)-positive human breast cancer cells (BT-474) and NK cells (NK-92-CD16) in the presence of undifferentiated hMADS or #hMADS cells (corresponding to models of preadipocytes and adipocytes, respectively) or their control media (Figure 1A).