The reliability of manual IHC evaluation of FKBPL staining with image analysis software to quantitatively score FKBPL cytoplasmic staining was assessed within 263 tumour cores from cohort I. The median automated scores tended to increase as the manual scoring groups increased, although there appeared to be considerable overlap between the automated and manual scores with reasonable ICCCs for both scorers (Supplementary Figure 2), suggesting reasonable agreement between automated and manual scoring. The gene discussed is FKBPL; the disease is neoplasm.