It is important to note that experiments conducted to investigate the role of EphA2 during the mid-phase of the chlamydial cycle were performed with a low MOI of 1–2 (in contrast to MOI of 50–100 for adherence and MOI of 20–50 for invasion experiments) to ensure similar initial infection irrespective of the EphA2 status (Infectivity assay-Fig 5A). This evidence concerns the gene EPHA2 and infection.