As with our previous study investigating DUX4-FL expression in large single cultures of myogenic cells from nine of the Wellstone Center cohorts (03, 07, 09, 12, 15, 16, 17, 18, 20) [33], for each of the three donor families (07, 09, 17), the average frequency of DUX4-FL-expressing nuclei was higher in differentiated cells from the FSHD donor than from the unaffected donor across multiple cultures (Table 1, n = 4 to 14); this difference reached significance (P < 0.05, t-test) in every case except 07Adel vs. 07Udel (P < 0.15) (Table 1). This evidence concerns the gene DUX4 and facioscapulohumeral muscular dystrophy.