MYC and penile carcinoma: We used a multi-fluorophore labelled, quantitative immunofluorescence [6] technique, in combination with high throughput and confocal imaging and unbiased fluorophore signal quantification, to investigate the expression and co-localization of Wnt signaling targets, WNT4, CD1, MMP7 and c-MYC in penile carcinoma and normal samples.