Similarly, in a phase IB study in pretreated advanced melanoma patients, vaccination with monocyte-derived DCs, electroporated with mRNA coding for melanoma-associated antigen combined with immunostimulatory RNAs (isRNAs) (i.e. CD40 ligand, TLR 4 and CD70), induced tumor associated antigen (TAA) CD8+ immunity [95,96]. This evidence concerns the gene CD40LG and melanoma.