ICC staining (Figure 2) showed that Notch1 and Notch2 as well as their downstream gene HES1 were expressed in OVCAR-3 and CAOV-3 cells; upon resveratrol treatment, Notch2 and HES1 expression were suppressed in both CAOV-3 and OVCAR-3 cells, while the expression levels of Notch1 was weakly increased in the former and decreased in the later cell line. This evidence concerns the gene HES1 and intrahepatic cholangiocarcinoma.