Similarly, the Western blot analysis indicated that the expressions of TGF-β, N-cadherin Vimentin, ZEB-1, SMAD-2, SMAD-3, SMAD-7, GLI1 and GLI2 were all decreased, too, and that the SMAD-7 and E-cadherin expression were markedly increased in the tumor tissues from the mice vaccinated with the B16F10/GPI-IL-21 vaccine and challenged by the B16F10/shTGF-β1 and the injection of miR200c agomir. The gene discussed is SMAD2; the disease is neoplasm.