Compared with those from other groups, the expression of TGF-β1, Vimentin, ZEB-1, N-cadherin, Gli1/2, and P-Smad2/3 was significantly decreased in the tumor areas from the mice vaccinated with B16F10/GPI-IL-21 combined with the B16F10/shTGF-β1 challenge and the administration of miR200c agomir. The gene discussed is GLI1; the disease is neoplasm.