Our data demonstrated that high level of IFN-γ and low level of TGF-β1 did reinforce antitumor effectiveness and result in being nearly free of the B16F10 melanoma as well as less metastatic focus in the lungs and lymph nodes in the mice that were received B16F10/GPI-IL-21 vaccine and challenged by the shTGF-β1-B16F10 cells along with the miR200c agomir administration. Here, IL21 is linked to melanoma.