In addition, the CD4+CD25+Treg cells derived from the splenocytes and tumor draining lymph nodes were significantly reduced in the tumor vaccine in combination with shTGF-β1 and miR200c group as shown in Figure 4D, 4E and 4F; these findings suggested that the multiple immune effects were induced through using the tumor vaccine B16F10/GPI-IL-21 combined with the down- regulated TGF-β1 and the administration of miR200c agomir. The gene discussed is TGFB1; the disease is neoplasm.