To further dissect the mechanism why infiltrated mast cells suppressed AR signaling could increase PCa cell invasion, we then applied focus-array with several PCa metastasis-related genes (Table S1) and found MMP9 was consistently increased in all four different PCa cell lines (with many other metastasis-related genes increased in one, two or three PCa cell lines, and qPCR and western blot assays confirmed the increased MMP9 at both mRNA (Figure 4A) and protein level (Figure 4B). This evidence concerns the gene MMP9 and posterior cortical atrophy.