In order to determine whether the upregulation of TWIST1/2 by MACC1 generally exists in the carcinogenesis and tumor progression of multiple cancers, we chose three poorly differentiated cancer cell lines, including human colon cancer cell lines (HCT-116 and SW480), breast cancer cell lines (MDA-MB-231 and BT549) and lung cancer cell lines (A549 and NCIH520). The gene discussed is TWIST1; the disease is neoplasm.