However, despite these developments, multiple questions remain regarding (i) the best method to screen for these mutations, (ii) the distinction between relevant and irrelevant mutations as well as the precise pathogenetic and phenotypic role of the known aberrations for each of the MPN subtypes, (iii) changes of subclones during treatment and (iv) the prognostic role of single vs. combined mutations (i.e. TP53 mutation alone or together with an IDH1 mutation). The gene discussed is IDH1; the disease is myeloproliferative disorder.