We have developed this assay for the nanoconjugate P/mPEG(5%)/LLL(40%)/Herceptin(0.2%)/AONHER2(2%) (P is denoted as the PMLA backbone; mPEG as methoxypolyethylene glycol; LLL as trileucine; % refers to feed composition as the fraction of total malic acid units in polymer), which is an effective growth inhibitor in mouse models of human Her2/neu positive breast tumor [15]. Here, ERBB2 is linked to breast neoplasm.