Metabolome analysis demonstrated an altered metabolite and amino acid content in VHL− vs. VHL+ cells (Figure S2), thus supporting their distinct metabolism and was further strengthened by recent work of Gameiro and co-authors describing reduced intracellular citrate levels in VHL mutant versus VHL wt RCC cells due to the presence of HIF thereby linking VHL and hypoxia and sensitizing VHL-deficient cells to glutamine deprivation [26]. This evidence concerns the gene VHL and renal cell carcinoma.