Furthermore, reduction of Mcl-1 and Sox2 in hepatoma cells by ψ-Bufarenogin was robustly blocked by DN-Akt (the dominant negative mutant Akt) [27] overexpression, indicating that PI3-K/Akt should be involved in ψ-Bufarenogin-facilitated apoptosis of hepatoma cells and the repression of hepatic T-ICs expansion. The gene discussed is SOX2; the disease is hepatocellular carcinoma.