This work showed that an hpRNAi construct containing sequences of the Rep, TrAP and REn of the monopartite begomovirus Cotton leaf curl Multan virus could prevent symptomatic infection by transient assay in N. benthamiana, but that in the presence of the cognate betasatellite, Cotton leaf curl Mutan betasatellite, a significant number of plants developed symptoms. The gene discussed is REN; the disease is infection.