NUMB and non-small cell lung carcinoma: They then went on to demonstrate, using an A549 non-small cell lung cancer (NSCLC) cell line with an RBM10v1(V354E) mutation (herein referred to as A549-JV), that expression of recombinant RBM10v1 protein, with either a valine or a glutamic acid at amino acid 354, altered NUMB splicing.