It is widely known that focal adhesion kinases are mediators of the integrin pathway and that its phosphorylation can be activated by αvβ3 integrin under hypoxia in glioma cells; therefore, we determined whether the inhibitory effect of melatonin on FAK and Pyk2 phosphorylation might be mediated through αvβ3 integrin. This evidence concerns the gene PTK2B and central nervous system cancer.