Thus, we demonstrated that mice acutely infected with high parasite loads exhibited a significant differences with respect to the parasitemia onset and peak and that increased colon width, intestinal inflammatory infiltration, myositis, periganglionitis, ganglionitis, pro-inflammatory cytokine production (TNF-α, INF-γ, IL-2, IL-6I, L-17) and amastigote nest formation was also observed mainly in animals infected with high parasitic loads. Here, TNF is linked to parasitic infectious disease.