The results of the present study show that the serum PINP, PICP, and CTX levels in the osteoporosis model group were significantly larger than those in the normal control group, while the combined extracts of Herba Epimedii and Fructus Ligustri Lucidi significantly decreased the serum levels of PINP, PICP, and CTX. This evidence concerns the gene CYP27A1 and osteoporosis.