Treatment of OS cells with 5-azaC induced expression of PTEN, an inhibitor of the PI3K/Akt pathway, while studies in pancreatic cancer demonstrate that 5-azaC treatment reduces proliferation of pancreatic cancer cells through inhibition of Wnt/β-catenin signaling and its downstream target c-myc [32,33]. Here, PTEN is linked to familial pancreatic carcinoma.