Coincident with altered expression of cell cycle regulators, the expression of Ki67, a cellular marker for proliferation, was also significantly increased in miR-135a-transfected cells, and decreased in miR-135a-inhibitor-transfected cells (Figure 4A–C), which further confirmed that miR-135a promoted the proliferation and cell cycle progression of bladder cancer cells. The gene discussed is MKI67; the disease is urinary bladder carcinoma.