The effects of TNF and IL-1β on two key NF-κB signaling molecules, p-IκB-α (Ser32) and p65 component (p-NF-κB (Ser536)), were assessed by Western blot analysis in WT, S59P, R92Q and T50M HEK-293, and in PBMCs obtained from TRAPS patients and controls. The gene discussed is NFKB1; the disease is TNF receptor 1-associated periodic fever syndrome.