For this, we investigated the expression of angiogenesis-induced proteins, such as VEGF and Ang-1/Tie-2, and tight junction molecules such as Occludin and ZO-1 with the BBB permeability in transient middle artery cerebral occlusion (tMCAO)-induced ischemic stroke in rats, and investigated its action mechanism on the PI3K/Akt signaling pathway. The gene discussed is VEGFA; the disease is ischemic stroke.