In this study, the hairy root extract of AG inhibits the brain infarction, edema, and BBB leakage in MCAO-induced ischemic rats through the inhibition of glial activation, and the increase of Ang-1, Tie-2, VEGF and tight junction proteins, ZO-1 and Occludin and the activation of PI3K/Akt. Here, OCLN is linked to brain infarction.