As the major effect of the activation of TLR7 is the induction of IFN-γ [18], we observed that when EL4 tumour cells were co-cultured with lymphocytes from the surviving mice treated by SZU-101, especially with the lymphocytes from the CoAd i.t. group, IFN-γ release was significantly increased (Figure 5A). This evidence concerns the gene IFNG and neoplasm.