In order to overcome this problem and recognize the role of cardiomyocyte β1-AR activation in cardiomyocyte apoptosis during endotoxemia, we first isolated adult mouse ventricular myocytes and investigated the effects of DOB, a β1-AR agonist, on LPS-induced cardiomyocyte apoptosis. Here, ADRB1 is linked to serum lipopolysaccharide activity.