ENO1 and non-small cell lung carcinoma: Further, ENO1 was observed to express in the cytoplasm but not in the nucleus in NSCLC A549 and SPCA-1 cells by immunofluorescence assay (Figure 1C), and its upregulated expression levels in mRNA and protein were also found in both two cells compared to immortalized human bronchial epithelial cell line HBE (Figure 1D).