In this experiment, we preliminary identified the relationship between tumor marker CA125 and ovarian cancer stem cells, and obtained satisfactory results The second antibody labeled by monoclonal antibody of CA125 (OC125) and APC was used to isolate tumor cells with CA125 expression, and lineage-specific antibodies labeled by PE, FITC and other fluorescence were used to eliminate human normal cells in the tumor tissues; PI was used to eliminate dead cells. This evidence concerns the gene APC and neoplasm.