In addition, we performed immunohistochemical (IHC) staining for pS6 at serine 235/236 (pS6Ser235/236, 1:150 dilution, rabbit monoclonal, Cell Signaling Techmology) and at serine 240/244 (pS6Ser240/244, 1:200 dilution, rabbit polyclonal, Cell Signaling Technology) and PTEN (1:50 dilution, rabbit monoclonal, Cell Signaling Technology) with tumor tissues obtained just before and 2 months after re-treatment with everolimus. The gene discussed is PTEN; the disease is neoplasm.