We first evaluated the effect of IVIg on the protein expressions of TLR2, TLR4 and TLR8 in a cell culture model of ischemic neuronal injury in which primary mouse cortical neurons were subjected to conditions mimicking ischemic stroke, including glucose deprivation (GD) and combined oxygen and glucose deprivation (OGD) (Figure 1). This evidence concerns the gene TLR4 and ischemic stroke.