The perturbations included activation (e.g., using a retrovirus to overexpress an oncogene) or silencing (e.g., an RNA interference experiment), and targeted many genes that are important in cancer, including breast cancer (e.g., ERBB2, TP53, MYC, AKT, RB1, CCND1, etc., see Additional file 1: Table S1). The gene discussed is RB1; the disease is breast carcinoma.