To determine if p53R172H exhibits gain-of-function properties in HCC, we generated Albumin-tva, LSL-Trp53R172H/flox, Albumin-cre mice (hereafter referred to as p53R172H mice) and Albumin-tva, Trp53flox/flox, Albumin-cre (p53fl/fl) littermates. Here, ALB is linked to hepatocellular carcinoma.