Apart from Li-7 cells, the HCC cell lines tested were relatively “homogeneous” in terms of the expression pattern of CD13 and CD166 than Li-7 cells, and only contained either CD13(+) CD166(+) (in HuH-7 and PLC/PRF/5 cells) or CD13(−)CD166(+) population (in HLE and HLF cells); they also displayed a “stable” population pattern during culture in vitro (Table 1). Here, ALCAM is linked to hepatocellular carcinoma.