In summary, the expression profiles of the primary lung cancer, adjacent benign lung tissue, and metastatic brain tissue from one patient were established using an RNA-Seq assay, and subsequent bioinformatic analyses demonstrated that the actinin gene ACTN4 and the pathways involved in the regulation of cytoskeleton organization, cell motility, and focal adhesion are indispensable for the process of lung cancer metastasis to the brain. The gene discussed is ACTN4; the disease is lung carcinoma.