We carried out a supervised clustering approach comparing three different groups of samples in a 2x2x2 study design; MCF-7 breast cancer cell line with IgG1 isotype control antibody, MCF-7 co-cultured with the two IFN-positive CAFs and the IgG1 antibody and lastly, MCF-7 co-cultured with the two IFN-positive CAFs and treated with the IFN-β neutralizing antibody. The gene discussed is IFNB1; the disease is breast cancer.