SPTB and preterm premature rupture of the membranes: RT-qPCR was used for validation of group PPROM compared to sPTB, PROM, and FTB; group sPTB compared to FTB and PROM; group PROM compared to FTB; the group of preterm labor [PPROM + sPTB] compared to full-term labor [PROM + FTB]; and the group of rupture of membrane [PPROM + PROM] compared to non-rupture of membrane [sPTB + FTB], respectively (Figure 3).