In this sense, the immunohistochemical criteria that we used for evaluation of S100A14 and S100A16 positivity in breast cancers, which evaluated the staining pattern and staining intensity of these proteins based on their subcellular localization and potential function in cytoskeletal organization may be a more suitable method for evaluation of these proteins than simply evaluation of total cellular expression of these proteins. This evidence concerns the gene S100A16 and breast cancer.