Co-expression of S100A14 and S100A16 was correlated with younger patient age (<60 years old,P= 0.025), lymph node metastasis (P= 0.005), ER-negative status (P= 0.008) and HER2-positive status (P < 0.001). Here, S100A16 is linked to metastatic malignant neoplasm in the lymph nodes.