SLC9A1 and infection: Our results indicated that infection of H9c2 cardiomyocytes or NRVMs with the NHE1 adenovirus successfully overexpressed the HA-tagged protein (S1A Fig and S2A Fig), as well as total NHE1 protein expression (S1B Fig and S2B Fig), an effect that was not observed in cardiomyocytes infected with the GFP adenovirus alone.