5xFAD mice overexpress (under control of Thy1 promoter) both mutant human APP with the Swedish (K670N, M671L), Florida (I716V), and London (V717I) familial Alzheimer's disease (FAD) mutations and human PSEN1 harboring two FAD mutations (M146L and L286V) [15]. Here, APP is linked to familial Alzheimer disease.