Finally, in order to determine the functionality of the VAMP1 polymorphisms, we employed a dual luciferase reporter gene assay to test the best two expression-associated polymorphisms (rs7390 and rs12964) and the best Alzheimer’s disease susceptibility polymorphism (rs2072376) in a human hepatocellular carcinoma line (HepG2). The gene discussed is VAMP1; the disease is early-onset autosomal dominant Alzheimer disease.