To further investigate why the non-transformed MCF10A cells behave differently compared to MCF7 and MDA MB 231 breast cancer cells in response to ACT1 treatment, we evaluated Cx43 intracellular distribution by IF and gap junction activity by gap-FRAP in MCF10A cells after R-pep and ACT1 treatment. The gene discussed is TRAF3IP2; the disease is breast cancer.