The method was successfully applied to CRC diagnosis by analyzing the expression of five genes (β-actin, C-myc, H-ras, CD44v6, Cox-2, and N-ras) and three mutation loci on APC genes (codons 1406, 1338, and 1356) in CRC tissues, CRC cells, and stool samples from CRC patients. This evidence concerns the gene NRAS and colorectal carcinoma.