The method was successfully applied to CRC diagnosis by analyzing the expression of five genes (β-actin, C-myc, H-ras, CD44v6, Cox-2, and N-ras) and three mutation loci on APC genes (codons 1406, 1338, and 1356) in CRC tissues, CRC cells, and stool samples from CRC patients. Here, HRAS is linked to colorectal carcinoma.