Using the Cox regression multivariate analysis, we found that PVTT, ELF expression, and TGF-β1 expression were the significant independent related factors for DFS (all P < 0.05), in addition, tumor differentiation (P = 0.029), PVTT (P = 0.011), ELF expression (P = 0.042) and TGF-β1 expression (P < 0.001) were the significant independent related factors for OS (Tables 5 and 6). This evidence concerns the gene TGFB1 and neoplasm.