Our results further validated that GSE at high concentrations (50–400 μg/ml) have high oxidative stress and apoptosis in terms of ROS generation, mitochondrial depolarization, annexin V/PI staining, and caspase activation but not for low concentrations (<10 μg/ml) of GSE in oral cancer Ca9-22 cells. Here, ANXA5 is linked to lip and oral cavity carcinoma.